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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - spectrophotometers. As drawn in Fig - http://www.video-bookmark.com/bookmark/6114703/olis-clarity/. 9, the response may also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this could be due to the fact that the H4B radical acts as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A model basics for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.